Stock Photo - HPLC. High-performance liquid chromatography. High-pressure liquid chromatography. Technique in analytic chemistry used to separate the components in a mixture, to identify each component, and to quantify each component. It relies on pumps to pass a pressurized liquid solvent containing the sample mixture through a column filled with a solid adsorbent material. Each component in the sample interacts slightly differently with the adsorbent material, causing different flow rates for the different components and leading to the separation of the components as they flow out the column. Laboratory. Energy and Environment Division. Tecnalia Research and Innovation. Donostia. San Sebastian. Gipuzkoa. Basque Country. Spain.